Universal Primer List | |
---|---|
Primer Name | Primer Sequence 5'-3' |
T7 Promotor | TAA TAC GAC TCA CTA TAG GG |
T7 Terminator | GCT AGT TAT TGC TCA GCG G |
T3 | ATT AAC CCT CAC TAA AG |
SP6 | GAT TTA GGT GAC ACT ATA G |
M13F(-21) | TGT AAA ACG ACG GCC AGT |
M13F(-40) | GTT TTC CCA GTC ACG AC |
M13R Reverse | CAG GAA ACA GCT ATG AC |
AOX1 Forward | GAC TGG TTC CAA TTG ACA AGC |
AOX1 Reverse | GCA AAT GGC ATT CTG ACA TCC |
pGEX Forward (GST 5, pGEX 5') | GGG CTG GCA AGC CAC GTT TGG TG |
pGEX Reverse (GST 3, pGEX 3') | CCG GGA GCT GCA TGT GTC AGA GG |
BGH Reverse | TAG AAG GCA CAG TCG AGG |
GFP (C' terminal, CFP, YFP or BFP) | CAC TCT CGG CAT GGA CGA GC |
GFP Reverse | TGG TGC AGA TGA ACT TCA GG |
GAG | GTT CGA CCC CGC CTC GAT CC |
GAG Reverse | TGA CAC ACA TTC CAC AGG GTC |
CYC1 Reverse | GCG TGA ATG TAA GCG TGA C |
pFastBacF | GGA TTA TTC ATA CCG TCC CA |
pFastBacR | CAA ATG TGG TAT GGC TGA TT |
pBAD Forward | ATG CCA TAG CAT TTT TAT CC |
pBAD Reverse | GAT TTA ATC TGT ATC AGG |
CMV-Forward | CGC AAA TGG GCG GTA GGC GTG |
LKO.1 5' (hU6 promoter) | GAC TAT CAT ATG CTT ACC GT |