Glucose Assay Kit I
Glucose is the most important carbohydrate in biology. Transported via the blood stream, it is the primary source of energy for the body’s cells. Glucose levels are highly regulated in the human body. Failure to maintain blood glucose in the normal range leads to conditions of persistently high or low blood sugar. Diabetes mellitus is the most prominent disease related to improper blood sugar regulation. The determination of glucose levels in blood is critical in the control of diabetes. Eton’s Glucose Assay Kit provides a simple, reproducible, and sensitive tool for assay glucose in plasma, serum, and urine. The glucose assay uses the glucose oxidase-peroxide reaction for the determination of glucose concentrations. The color intensity of the reaction product at 490nm is directly proportional to the glucose concentration in the sample. The kit is stable until the expiration date under proper storage and handling conditions. The product is for research only.
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Sample Typle |
Plasma,Serum,Cell culture supernatant, other body fluid |
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Contents |
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| Method | Colorimetric method at 490nm |
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Standard Curve |
 |
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Sensitivity |
8µM-400µM |
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Reaction Time |
15 minutes |
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Applications |
For biological research: |
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Storage |
-80°C |
| Notes |
Materials needed but not supplied A plate reader capable of measuring absorbance at 490nm Adjustable pipettes and a repeat pipettor Distilled water(milliQ or HPLC-grade) 0.5M Acetic Acid Clear flat bottom 96-well plates if not included in the kit purchased |
| Shipping | Icepacks |
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Reagent Preparation Note: All reagents are frozen. We recommend you spin the standard vial before opening. 1. Glucose Standard(2x) Note: Please don’t do dilution in the provided Glucose standard vial itself. The vial contains 500µl of 800µM Glucose Standard. The standard must be equilibrated to room temperature before use. Dilute 500µl of 800µM Glucose Standard with 500µl of dH2O to prepare a 400μM Glucose Standard. 1ml of diluted standard is enough for making 3 standard curves if assayed in duplicate. Store at -80?C. 2. Glucose Assay Solution The solution contains enzymes that are light sensitive. The solution must be thawed on ice before use.Best to aliquot the amount needed and use it all to prevent thawing/freezing cycles. Freeze and store aliquots at -80 oC |
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Protocol 1.Sample Preparation Serum/Plasma/other body fluid/cell culture supernatant Note: |
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2.Standard Curve Prepation We recommend that Glucose Standards be assayed in duplicate. A standard curve has to be run in each assay. |
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3.Perform the assay |
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4. Calculation a. Average the OD490 nm values of replicate wells of each Glucose standard, test samples, and blank. In order to get the corrected absorbance, subtract the average OD490 nm value of the blank from the averaged OD490 nm values from all standards and samples. Glucose(μM)= [ (Corrected absorbance)-(y-intercept)]/Slope |
Material Safety Data Sheet
Date:Sept 21,2017
1. PRODUCT AND COMPANY IDENTIFICATION
1.1 Product identifier
Product name: Glucose Assay Kit I
Catalog No: 1200031002, 120003100A, 120003100P, 1200032002, 120003200A, 12003200P, 1200034002, 120003400A, 120003400P
1.2 Relevant identified uses of the substance or mixture and uses advised against
For research use only.
1.3 Details of the supplier of the safety data sheet
Company:Eton Bioscience Inc
TollFree:1-800-758-1630
Tel:1-800-758-1630
Fax:1-800-507-2912
1.4 Emergency telephone number
1-800-758-1630
2. HAZARDS IDENTIFICATION
2.1 Classification of the substance or mixture
Not a hazardous substance or mixture
2.2 GHS Label elements, including precautionary statements
Not a hazardous substance or mixture
2.3 Other hazards
None
3. COMPOSITION/INFORMATION ON INGREDIENTS
3.1 Substances
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Glucose Assay Solution |
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Components |
CAS# |
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Potassium phosphate monobasic |
7778-77-0 |
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Potassium phosphate dibasic |
7758-11-4 |
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Glucose Oxidase |
9001-37-0 |
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Glucose Assay Standard |
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Components |
CAS# |
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Sodium D-Glucose |
50-99-7 |
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Potassium phosphate monobasic |
7778-77-0 |
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Potassium phosphate dibasic |
7758-11-4 |
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Water |
7732-18-5 |
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4. FIRST AID MEASURES
4.1 Description of first aid measures
Eye contact
Check for and remove contact lenses and flush with copious amounts of water; assure adequate flushing by separating the eyelids with fingers; call a physician
Skin Contact
Flush with copious amounts of water; remove contaminated clothing and shoes; call a physician
Inhalation
Remove to fresh air. If not breathing give artificial respiration
Ingestion
If swallowed, never give anything by mouth to an unconscious person. Rinse mouth with water.
4.2 Most important symptoms and effects, both acute and delayed
No information available
4.3 Indication of any immediate medical attention and special treatment needed.
No information available
5. FIRE FIGHTING MEASURES
5.1 Extinguishing media
Suitable extinguishing media
Water spray, alcohol-resistant foam, dry chemical or carbon dioxide
5.2 Special hazards arising from the substance or mixture
No information available
5.3 Advice for firefighters
Wear self-contained breathing apparatus and protective clothing to prevent contact with skin and eyes.
6. ACCIDENTAL RELEASE MEASURES
6.1 Personal precautions,protective equipment and emergency procedures
Use full personal protective equipment. Evacuate personnel to safe areas. Avoid breathing vapors,mist or gas.
6.2 Environmental precautions
Prevent further leakage or spillage. Prevent product from entering drain.
6.3 Methods and material for containment and cleaning up
Keep in suitable, closed containers for disposal
7. HANDLING AND STORAGE
7.1 Precautions for safe handling
Avoid skin/eye contact. Use protective equipment as needed. Wash contaminated clothing before reuse.
7.2 Conditions for safe storage,including any incompatibilities
Keep container tightly closed. Store at -80?C.
7.3 Specific end use(s)
No data available.
8. EXPOSURE CONTROLS/PERSONAL INFORMATION
8.1 Control Parameters
This product contains no hazardous materials with occupational exposure limits.
8.2 Exposure controls
Engineering controls
Provide shower and eye wash station
Personal protective equipment
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Eye Protection |
Wear safety goggles |
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Skin Protection |
Wear protective clothing |
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Hand Protection |
Wear protective gloves |
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Respiratory protection |
Wear NIOSH/MSHA approved respirators |
9. PHYSICAL AND CHEMICAL PROPERTIES
9.1 Information on basic physical and chemical properties
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Appearance |
Clear, colorless liquid |
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pH |
7.0 |
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Melting point |
No data available |
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Freezing point |
No data available |
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Boiling point |
No data available |
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Density |
No data available |
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Water solubility |
Soluble |
9.2 Other safety information
No data available.
10. STABILITY AND REACTIVITY
10.1Reactivity
No data available
10.2Chemical stability
Stable under recommended storage conditions.
10.3Possibility of hazardous reactions
No data available.
10.4Conditions to avoid
No data available
10.5Incompatible materials
Strong oxidizing agents
10.6Hazardous decomposition products
Hazardous decomposition products formed under fire conditions: carbon oxides
11. TOXICOLOGICAL INFORMATION
11.1Information on toxicological effects
Toxicological effects on this product have not been thoroughly studied.
12. ECOLOGICAL INFORMATION
12.1Toxicity
Avoid release into environment
12.2Persistence and degradability
No data available
12.3.Bioaccumulative potential
No data available
12.4Mobility in soil
No data available
12.5Results of PBT and vPvB assessment
No data available
12.6Other adverse effects
No data available
13. DISPOSAL INFORMATION
13.1Waste treatment methods
Dispose in accordance with local, state, and federal regulations.
14. TRANSPORT INFORMATION
DOT:
Proper shipping name: none
Non-Hazardous for transport: this substance is considered to be non-hazardous for transport
IATA:
Proper shipping name: none
Non-Hazardous for transport: this substance is considered to be non-hazardous for transport
ADR/RID
Proper shipping name: none
Non-Hazardous for transport: this substance is considered to be non-hazardous for transport
15. REGULATORY INFORMATION
EU Risk and Safety phrases:
R36/37/38: irritating to eyes/respiratory system/skin
16. OTHER INFORMATION
The information above is believed to be accurate and represents the best information currently available to us. However, we make no warranty of merchantability or any other warranty, express or implied, with respect to such information. Eton Bioscience Inc. shall not be held liable for any damages or other consequences resulting from handling or from contact with the above product.