L-Lactate Assay Kit I
Lactate dehydrogenase converts lactate and NAD+ into pyruvate and NADH. The Lactate Assay Kit is based on the reduction of the terazolium salt INT in a NADH-coupled enzyme reaction to formazan, which exhibits an absorbance maximum at 490 nm. The intensity of the absorbance is proportional to the lactate concentration. Using a set of lactate standards, the assay can measure the concentration of lactate in samples (plasma, medium, or others) in a quantitative manner. The kit is stable until the expiration date under proper storage and handling conditions. The product is for research only.
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Sample Typle |
Plasma,Serum,Cell culture supernatant, other body fluid |
| Contents | 
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Method |
Colorimetric method at 490nm |
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Standard Curve |
 |
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Sensitivity |
60µM-3000µM |
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Reaction Time |
30 minutes |
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Applications |
For biological research: |
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Storage |
-80°C |
| Notes |
Materials needed but not supplied A plate reader capable of measuring absorbance between 470-490nm Adjustable pipettes and a repeat pipettor A source of pure water; glass distilled water of HPLC-grade water is sufficient 0.5M Acetic Acid Clear flat bottom 96-well plates if not included in the kit purchased |
| Shipping | Icepacks |
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Reagent Preparation Note: All reagents are frozen. We recommend you spin small vials before opening.
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Protocol 1.Sample Preparation Serum/Plasma/other body fluid/cell culture supernatant Note: |
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2.Standard Curve Preparation |
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3.Perform the assay a) Add 50ul of L-Lactate assay solution to each well containing L-Lactate standards and test samples. |
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4.Calculations a) Average the OD490 nm values of replicate wells of each L-Lactate standard, test samples, and blank. In order to get the corrected absorbance, subtract the average OD490 nm value of the blank (L-Lactate Standard #8) from the average OD490 nm values from all standards and samples. b) Make a standard curve by plotting OD490 nm values from each L-Lactate standards as a function of L-Lactate concentration. This can be done with excel spreadsheet. Calculate the value of L-Lactate in samples using the equation obtained from the linear regression of the standard curve.
L-Lactate(µM)= [ (Corrected absorbance)-(y-intercept)]/slope
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Material Safety Data Sheet
Date:Sept 21,2017
1. PRODUCT AND COMPANY IDENTIFICATION
1.1 Product identifier
Product name: L-Lactate Assay Kit I
Catalog No: 1200011002, 120001100A, 120001100P, 1200012002, 120001200A, 12001200P, 1200014002, 120001400A, 120001400P
1.2 Relevant identified uses of the substance or mixture and uses advised against
For research use only.
1.3 Details of the supplier of the safety data sheet
Company:Eton Bioscience Inc
TollFree:1-800-758-1630
Tel:1-800-758-1630
Fax:1-800-507-2912
1.4 Emergency telephone number
1-800-758-1630
2. HAZARDS IDENTIFICATION
2.1 Classification of the substance or mixture
Not a hazardous substance or mixture
2.2 GHS Label elements, including precautionary statements
Not a hazardous substance or mixture
2.3 Other hazards
None
3. COMPOSITION/INFORMATION ON INGREDIENTS
3.1 Substances
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L-lactate Assay Solution |
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Components |
CAS# |
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DMSO |
67-68-5 |
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L-LDH |
9001-60-9 |
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Tris-HCl,pH7.5 |
N/A |
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Water |
7732-18-5 |
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L-Lactate Assay Standard |
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Components |
CAS# |
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Sodium L-Lactate |
867-56-1 |
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Tris-HCl,pH7.5 |
N/A |
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Water |
7732-18-5 |
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4. FIRST AID MEASURES
4.1 Description of first aid measures
Eye contact
Check for and remove contact lenses and flush with copious amounts of water; assure adequate flushing by separating the eyelids with fingers; call a physician
Skin Contact
Flush with copious amounts of water; remove contaminated clothing and shoes; call a physician
Inhalation
Remove to fresh air. If not breathing give artificial respiration ;call a physician
Ingestion
If swallowed, never give anything by mouth to an unconscious person. Rinse mouth with water ;call a physician
4.2 Most important symptoms and effects, both acute and delayed
No information available
4.3 Indication of any immediate medical attention and special treatment needed.
No information available
5. FIRE FIGHTING MEASURES
5.1 Extinguishing media
Suitable extinguishing media
Water spray, alcohol-resistant foam, dry chemical or carbon dioxide
5.2 Special hazards arising from the substance or mixture
No information available
5.3 Advice for firefighters
Wear self-contained breathing apparatus and protective clothing to prevent contact with skin and eyes.
6. ACCIDENTAL RELEASE MEASURES
6.1 Personal precautions,protective equipment and emergency procedures
Use full personal protective equipment. Evacuate personnel to safe areas. Avoid breathing vapors,mist or gas.
6.2 Environmental precautions
Prevent further leakage or spillage. Prevent product from entering drain.
6.3 Methods and material for containment and cleaning up
Keep in suitable, closed containers for disposal
7. HANDLING AND STORAGE
7.1 Precautions for safe handling
Avoid skin/eye contact. Use protective equipment as needed. Wash contaminated clothing before reuse.
7.2 Conditions for safe storage,including any incompatibilities
Keep container tightly closed. Store at -80?C.
7.3 Specific end use(s)
No data available.
8. EXPOSURE CONTROLS/PERSONAL INFORMATION
8.1 Control Parameters
This product contains no hazardous materials with occupational exposure limits.
8.2 Exposure controls
Engineering controls
Provide shower and eye wash station
Personal protective equipment
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Eye Protection |
Wear safety goggles |
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Skin Protection |
Wear protective clothing |
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Hand Protection |
Wear protective gloves |
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Respiratory protection |
Wear NIOSH/MSHA approved respirators |
9. PHYSICAL AND CHEMICAL PROPERTIES
9.1 Information on basic physical and chemical properties
L-Lactate Assay Solution
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Appearance |
Liquid, yellowish |
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pH |
7.5 |
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Melting point |
No data available |
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Freezing point |
No data available |
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Boiling point |
No data available |
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Density |
No data available |
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Water solubility |
Soluble |
L-Lactate Standard
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Appearance |
Liquid, colorless |
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pH |
7.5 |
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Melting point |
No data available |
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Freezing point |
No data available |
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Boiling point |
No data available |
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Density |
No data available |
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Water solubility |
Soluble |
9.2 Other safety information
No data available.
10. STABILITY AND REACTIVITY
10.1Reactivity
No data available
10.2Chemical stability
Stable under recommended storage conditions.
10.3Possibility of hazardous reactions
No data available.
10.4Conditions to avoid
No data available
10.5Incompatible materials
Strong oxidizing agents
10.6Hazardous decomposition products
Hazardous decomposition products formed under fire conditions: carbon oxides
11. TOXICOLOGICAL INFORMATION
11.1Information on toxicological effects
Toxicological effects on this product have not been thoroughly studied.
12. ECOLOGICAL INFORMATION
12.1Toxicity
Avoid release into environment
12.2Persistence and degradability
No data available
12.3.Bioaccumulative potential
No data available
12.4Mobility in soil
No data available
12.5Results of PBT and vPvB assessment
No data available
12.6Other adverse effects
No data available
13. DISPOSAL INFORMATION
13.1Waste treatment methods
Dispose in accordance with local, state, and federal regulations.
14. TRANSPORT INFORMATION
DOT:
Proper shipping name: none
Non-Hazardous for transport: this substance is considered to be non-hazardous for transport
IATA:
Proper shipping name: none
Non-Hazardous for transport: this substance is considered to be non-hazardous for transport
ADR/RID
Proper shipping name: none
Non-Hazardous for transport: this substance is considered to be non-hazardous for transport
15. REGULATORY INFORMATION
EU Risk and Safety phrases:
R36/37/38: irritating to eyes/respiratory system/skin
16. OTHER INFORMATION
The information above is believed to be accurate and represents the best information currently available to us. However, we make no warranty of merchantability or any other warranty, express or implied, with respect to such information. Eton Bioscience Inc. shall not be held liable for any damages or other consequences resulting from handling or from contact with the above product.
Q. Can Ethanol interfere with the enzyme?
A. The enzyme can tolerate up to 20% of ethanol without affecting it’s enzymatic activity with L-Lactate.
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Marina Ostroukhova, Nicholas Goplen, Md Zunayet Karim, Lidia Michalec, Lei Guo, Qiaoling Liang, and Rafeul Alam.:The role of low-level lactate production in airway inflammation in asthma.Am J Physiol Lung Cell Mol Physiol, Feb 2012; 302: L300 - L307.