Description: TurboLigationTM Kit enables quick ligation of both blunt-end or cohesive-end DNA fragments in just 5 minutes at room temperature. The kit combines TurboLigaseTM, an ultra-pure recombinant T4 DNA Ligase, and 5X TurboLigation Buffer, an optimized quick ligation buffer. A 5 minute TurboLigation reaction offers the same efficiency as a traditional 16oC overnight ligation reaction. The reaction product is ready for bacterial transformation without any further treatment.
- Adding linkers or adapters to DNA
- Blunt or cohesive end ligation of duplex DNA
- DNA cloning
TurboLigaseTM: 5 unit/µl in Storage Buffer of 10 mM Tris-HCl, pH 7.5, 50 mM KCl, 0.1 mM EDTA, 1 mM dithiothreitol, and 50% glycerol
5X TurboLigation Buffer: 250 mM Tris-HCl, pH 7.5, 50 mM MgCl2, 5 mM dithiothreitol, 5 mM ATP, 25% polyethylene glycol 8000
Stability and Storage:
TurboLigase and 5X TurboLigation Buffer are stable at -20oC for 12 months from date of receipt.
Store TurboLigase and 5X TurboLigation Buffer at -20oC.
Shipped in gel packs.