TurboLigation Kit

Description: TurboLigationTM Kit enables quick ligation of both blunt-end or cohesive-end DNA fragments in just 5 minutes at room temperature. The kit combines TurboLigaseTM, an ultra-pure recombinant T4 DNA Ligase, and 5X TurboLigation Buffer, an optimized quick ligation buffer. A 5 minute TurboLigation reaction offers the same efficiency as a traditional 16oC overnight ligation reaction. The reaction product is ready for bacterial transformation without any further treatment.

Sku# Product Name Product Size Price QTY

Sku#

8000011001

Product Name

TurboLigation Kit 100 reactions

Product Size

100 reactions

Price

$167.48 USD

QTY

Sku#

8000011005

Product Name

TurboLigation Kit 500 reactions

Product Size

500 reactions

Price

$630.63 USD

QTY

Add to Cart

Applications:
  • Adding linkers or adapters to DNA
  • Blunt or cohesive end ligation of duplex DNA
  • DNA cloning
Components:
TurboLigaseTM: 5 unit/µl in Storage Buffer of 10 mM Tris-HCl, pH 7.5, 50 mM KCl, 0.1 mM EDTA, 1 mM dithiothreitol, and 50% glycerol
5X TurboLigation Buffer: 250 mM Tris-HCl, pH 7.5, 50 mM MgCl2, 5 mM dithiothreitol, 5 mM ATP, 25% polyethylene glycol 8000

Stability and Storage:
TurboLigase and 5X TurboLigation Buffer are stable at -20oC for 12 months from date of receipt.
Store TurboLigase and 5X TurboLigation Buffer at -20oC.
Shipped in gel packs.

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