Recombinant e.coli Fpg
Eton Bio Recombinant E. coli Formamidopyrimidine-DNA Glycosylase/Fpg is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Lys269) of E. coli Fpg.
Fpg is a DNA glycosylase that releases damaged bases preferentially from duplex DNA. It has an associated class I AP (apurinic/apyrimidinic) lyase activity.Fpg Cleaves the DNA backbone to generate a single-strand break at the site of the removed base, The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving 3' and 5' phosphoryl groups.
|Names||Formamidopyrimidine-DNA Glycosylase, Fapy-DNA Glycosylase, DNA-(Apurinic or Apyrimidinic Site) Lyase MutM, AP Lyase MutM, mutM, fpg|
|Formulation||Supplied as a 0.2 μM filtered solution of 20mM Tris-HCl, 100mM NaCl, 1mM EDTA, 1mM DTT, 50% Glycerol, pH 7.8|
|Shipping||The product is shipped on dry ice/ice packs.
|Storage||Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.
|Purity||Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
|Endotoxin||Less than 0.1 ng/μg (1 IEU/μg).|
|Amino Acid Sequence||
MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRLSDQPVLSVQRRAKYLLLELPE GWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKVLRYTDPRRFGAWLWTKELEGHNVLTHLG PEPLSDDFNGEYLHQKCAKKKTAIKPWLMDNKLVVGVGNIYASESLFAAGIHPDRLASSLSLAEC ELLARVIKAVLLRSIEQGGTTLKDFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRA TFYCRQCQKLEHHHHHH