Recombinant E.coli Lon Protease

Eton Bio Recombinant E. coli Lon Protease is produced by E.coli transformed with a plasmid containing sequence (Asn2-Lys784) of E. coli Lon Protease fused with a polyhistidine tag at the C-terminus.

Sku #Product NameProduct SizePriceQTY
3703220101 Recombinant E.coli Lon Protease 10?g 10?g $165.00 USD
3703220501 Recombinant E.coli Lon Protease 50?g 50?g $375.00 USD
3703225001 Recombinant E.coli Lon Protease 500μg 500μg $1600.00 USD
3703220012 Recombinant E.coli Lon Protease 1mg 1mg $2260.00 USD

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Lon Protease, is a member of the Lon protease family. They are found in archaea, bacteria and eukaryotes. Lon protease is ATP-dependent serine protease that mediates the selective degradation of mutant and abnormal proteins as well as certain short-lived regulatory proteins, including some antitoxins. It required for cellular homeostasis and for survival from DNA damage and developmental changes induced by stress. It degrades polypeptides processively to yield small peptide fragments that are 5 to 10 amino acids long and binds to DNA in a double-stranded, site-specific manner. Endogenous substrates include the regulatory proteins RcsA and SulA, the transcriptional activator SoxS, and UmuD. Its overproduction specifically inhibits translation through at least two different pathways, one of them being the YoeB-YefM toxin-antitoxin system.

Source E. coli
Names Lon Protease, ATP-Dependent Protease La, lon, capR, deg, lopA, muc
Accession # P0A9M0
Formulation Supplied as a 0.2 μM filtered solution of 50mM Tris-HCl, 100mM KCl, 10% Glycerol, pH 7.5
Shipping The product is shipped on dry ice/ice packs.
Storage Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.
Purity Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
Endotoxin Less than 0.1 ng/µg (1 IEU/µg).
Amino Acid Sequence

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