Mammalian Cell Expression Service:

Eton now offers custom gene cloning, expression and stable cell-line development service. Using your desired vectors and clonal selection methods, we can isolate and express the desired gene for you and establish clonal cell-lines with the highest levels of target gene expression. This service includes:

• Isolation of gene of interest;
• Generation of recombinant construct;
• Verification of recombinant construct by restriction mapping and sequence analysis;
• Preparation of plasmid construct DNA;
• Introduce plasmid DNA into mamalian cells;
• Selection of over-expressing cells;
• Small scale expression and purification study assessed by SDS-PAGE and Western blot;
• Estimation of protein yield;
• Developing of stable cell line and expression of gene of interest.