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TEV Protease (TurboTEV) Ultra-pure TEV protease. Robust activity, high specificity, easy to remove from protein samples. TEV protease, TurboTEV, contains an enhanced form of a catalytic fragment of the N1a protein of Tobacco etch virus (TEV), a cysteine protease that recognizes the cleavage site of Glu-Asn-Leu-Tyr-Phe-Gln-Gly and cleaves between Gln and Gly. TurboTEV Protease is a restriction grade protease that has a robust activity at 4oC with high specificity and great stability. It does not require any special buffer for its activity and can be used in a buffer most suitable for the target protein. TurboTEV Protease is a 52 kDa protein with both GST and His tags so it can be removed by either Ni-chelating or Glutathione (GSH) resin. To order, please select the product below and click 'Add to Cart': |
Protein Type:
Protease
Source:
E. coli
Method of Purification:
Ni-NTA; GSH-4B.
Storage/Handling:
< - 20C
2 mg/ml in 25 mM Tris-HCl, pH8.0, 50 mM NaCl, 1 mM TCEP, 50% glycerol
A 49 kDa GST-fusion protein (C) at 1 mg/ml is incubated with TurboTEV or TEV Protease at a ratio of (1) 1:50, (2) 1:100, (3) 1:200 (w/w) in a buffer of 25 mM Tris-HCl, pH8.0, 150 mM NaCl, 14 mM b-mercaptoethanol at 4oC for 16 hours. The cleaved products are 27 kDa and 22 kDa. 'TEV' is a competitors’ TEV Protease product.
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