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HRV 3C Protease Ultra-pure HRV3C protease. Robust activity to cleave at the PreScission site with high specificity. His and GST-tags make it easy to remove from protein samples. Human rhinovirus 3C protease (HRV3C Protease) is a cysteine protease that recognizes the cleavage site of Leu-Glu-Val-Leu-Phe- Gln/Gly-Pro, commonly referred to as the PreScission Site. It cleaves between Gln and Gly. The recombinant form of the HRV3C protease is a restriction grade protease that has robust activity at 4oC and high specific activity. This HRV3C Protease is a 47 kDa protein with both GST and His tags so it can be removed by either Ni chelating or Glutathione (GSH) resin. To order, please select the product below and click 'Add to Cart': |
Protein Type:
Protease
Source:
E. coli
Method of Purification:
Ni-NTA; GSH-4B.
Storage/Handling:
< - 20C
2 mg/ml in 50 mM Tris-HCl, pH8.0, 150 mM NaCl, 1 mM EDTA, 1 mM TCEP, 50% glycerol
A 68 kDa GST-fusion protein (C) at 1 mg/ml is incubated with HRV3C Protease (*) at a ratio of (1) 1:50, (2) 1:100, (3) 1:200, (4) 1:400 (w/w) in a buffer of 25 mM Tris-HCl, pH8.0, 150 mM NaCl, 14 mM b-mercaptoethanol at 4oC for 16 hours. The cleaved products are 42 kDa and 26 kDa.
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