One unit of TurboNuclease converts 1.0 OD260 of salmon sperm DNA into acid-soluble nucleotides in 30 minutes at 37°C in a reaction buffer of 50 mM Tris-HCl, pH 8.0 and 1 mM MgCl2. This corresponds to complete digestion of 50 mg of salmon sperm DNA into oligonucleotides.
TurboNuclease has a specific activity of >1.3x106 units/mg. This is equivalent to >3x106 Kunitz units/mg, over 100-fold specific activity of most highly purified bovine DNase I (~25,000 Kunitz units/mg).
Figure 1. 50 ml of salmon sperm DNA was incubated with the indicated units of TurboNuclease and another brand of nuclease at 37oC for 30 min in a buffer of 50 mM Tris-HCl, pH 8.0 and 1 mM MgCl2. DNA digestion was monitored by agarose gel.
TurboNuclease shows no detectable protease activity.

TurboNuclease: 250 units/ml in 50 mM Tris-HCl, pH 8.0, 50 mM NaCl, 5 mM MgCl2 and 50% Glycerol.
TurboNuclease is purified through a proprietary process that achieves purity of >99% as shown in Figure 2.

Store TurboNuclease at -20oC. TurboNuclease can be flash-frozen in liquid nitrogen and stared at -70oC without loss of activity.

TurboNuclease can be used to reduce viscosity of cell lysates and remove nucleic acid contamination from sample preparations. It may reduce or prevent clumping of concentrated cells and frozen cells following thawing. TurboNuclease also replaces crude DNase I in many applications.
To reduce viscosity of cell lysate, 10-1000 units of TurboNuclease can be used for each gram of cell paste. The efficiency of viscosity reduction may vary with buffers, cell types, and cell lysis methods used. Due to its high specificity, the total amount TurboNuclease added is less than 1 mg/ml of lysate and will not complicate any down stream process.