L-Lactate Assay Kit I

Lactate dehydrogenase converts lactate and NAD+ into pyruvate and NADH. The Lactate Assay Kit is based on the reduction of the terazolium salt INT in a NADH-coupled enzyme reaction to formazan, which exhibits an absorbance maximum at 490 nm. The intensity of the absorbance is proportional to the lactate concentration. Using a set of lactate standards, the assay can measure the concentration of lactate in samples (plasma, medium, or others) in a quantitative manner. The kit is stable until the expiration date under proper storage and handling conditions. The product is for research only.

Product Name L-Lactate Assay Kit I (5 ml)-100 Assays- Assay Solution ONLY
SKU Number 120001100A
Product Size (5ml) - 100 assays- ASSAY SOLUTION ONLY
List Price $98.00 USD
  Quantity    
Product Name L-Lactate Assay Kit I (5ml) - 100 assays
SKU Number 1200011002
Product Size (5ml) - 100 assays
List Price $108.00 USD
  Quantity    
Product Name L-Lactate Assay Kit I (5 ml)-100 Assays w/Reader Plate (1x)
SKU Number 120001100P
Product Size (5ml) - 100 assays
List Price $120.00 USD
  Quantity    
Product Name L-Lactate Assay Kit I (10 ml)-200 Assays- Assay Solution ONLY
SKU Number 120001200A
Product Size (10ml) - 200 assays- ASSAY SOLUTION ONLY
List Price $146.00 USD
  Quantity    
Product Name L-Lactate Assay Kit I (10ml) - 200 assays
SKU Number 1200012002
Product Size (10ml) - 200 assays
List Price $156.00 USD
  Quantity    
Product Name L-Lactate Assay Kit I (10ml)-200 Assays w/ Reader Plate(2x)
SKU Number 120001200P
Product Size (10ml) - 200 assays
List Price $180.00 USD
  Quantity    
Product Name L-Lactate Assay Kit I (20ml) - 400 assays- ASSAY SOLUTION ONLY
SKU Number 120001400A
Product Size (20ml) - 400 assays- ASSAY SOLUTION ONLY
List Price $267.00 USD
  Quantity    
Product Name L-Lactate Assay Kit I (20ml) - 400 assays
SKU Number 1200014002
Product Size (20ml) - 400 assays
List Price $277.00 USD
  Quantity    
Product Name L-Lactate Assay Kit I (20ml)-400 Assays w/ Reader Plate(4x)
SKU Number 120001400P
Product Size (20ml) - 400 assays
List Price $326.00 USD
  Quantity    

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Sample Typle

Plasma,Serum,Cell culture supernatant, other body fluid

Components

Method

Colorimetric method at 490nm

Standard Curve

Sensitivity

50µM-3200µM

Reaction Time

30 minutes

Applications

For biological research:
L-Lactate measurement in biological samples
For drug/pharm research:
Drug influence on L-Lactate metabolism

Storage

-80°C

Shipping Icepacks


1

Prepare Standard Curve

2

Prepare Samples

3

Prepare Reaction Solution

4

Incubation for 30 minutes at 37°C

5

Read colorimetric absorbance at 490nm in a plate reader

6 Calculate concentration of your sample

MATERIAL SAFETY DATA SHEET
TRADE NAME: L-Lactate Assay Kit I
DATE OF ISSUE: Sept 18,2014
SKU#: 12001100A.1200011002, or 120001100P(100 assays), 120001200A,1200012002, or 120001200P (200 assays), 120001400A.1200014002, or 120001400P (400 assays)

SECTION I:PRODUCT AND MANUFACTURER INFORMATION

TRADE NAME:L-Lactate Assay Kit I
Manufacturer: Eton Bioscience Inc., 5820 Oberlin Drive, Suite 108, San Diego, CA 92121
Information Telephone:800.758.1630
Emergency Telephone: 800.758.1630

SECTION II: COMPOSITION / INFORMATION ON INGREDIENTS

CHEMICAL CHARACTERIZATION:
L-Lactate Assay Kit Solution:
CAS#

L-Lactate Assay Standard
CAS#
DMSO
67-68-5

Sodium L-Lactate
867-56-1
L-LDH
9001-60-9

Tris-HClpH7.5
N/A
Water
7732-18-5

Water
7732-18-5

SECTION III:HAZARD IDENTIFICATION

Potential harmful if any component is swallowed.
May cause irritation if contacted with skin or eyes. In such a case, flush skin or eyes with water for 15 min. Get medical attention if necessary

SECTION IV:FIRST AID MEASURES

General Information: Remove and wash contaminated clothing promptly.
IN CASE OF INHALATION:Supply fresh air and then consult a doctor.
IN CASE OF EYE CONTACT:Immediately flush eyes with copious amounts of water for 15 minutes.
IN CASE OF SKIN CONTACT:Immediately wash skin with soap and copious amounts of water.
IF SWALLOWED:Rinse mouth with copious amounts of water. Then consult a doctor.

SECTION V:FIREFIGHTING MEASURES

Potential harmful if any component is swallowed. May cause irritation if contacted with skin or eyes. In such a case, flush skin or eyes with water for 15 min.
Get medical attention if necessary

SECTION VI:ACCIDENTAL RELEASE MEASURES

PRECAUTIONARY MEASURES: No special measures required.
CLEAN-UP PROCEDURES: No special measures required.

SECTION VII:HANDLING AND STORAGE

HANDLING:
Exposure Limit: Undetermined;
Toxicity: We are not aware of any toxicity data for this product;
Carcinogenicity: Unknown;
Exposure symptoms: Unkown, handle with care;
Handling: Gloves, protective clothing and eyewear should be worn and safe laboratory practices followed;
In case of spill: Use appropriate protective equipment and methods to clean up spilled substance promptly. Absorb onto an appropriate material. Collect and dispose of all waste in accordance with applicable laws.
STORAGE: Keep tightly closed. Store at -80oC freezer.

SECTION VIII:EXPOSURE CONTROLS / PERSONAL PROTECTION

The routine precautionary measures for handling chemicals should be followed such as wearing chemical safety goggles, rubber gloves, rubber boots,
NIOSH / MSHA-Approved respirator when handling chemicals and wash thoroughly after handling.
Handle chemicals with care and avoid contact with eyes, skin, or on clothing.Use safety shower and eye bath as first aid measures if exposed to chemicals.

SECTION IX:PHYSICAL AND CHEMICAL PROPERTIES

L-Lactate Assay Solution

PHYSICAL STATE / FORM: Liquid
COLOR: Yellowish
ODOR:NONE
pH FACTOR:(20°C)around 7
VISCOSITY:(20°C)N/A
MELTING POINT:N/A
BOILING POINT:N/A
IGNITION TEMPERATURE:N/A
FLASHPOINT:N/A
EXPLOSION LEVEL:LOWER: N/A
UPPER: N/A
VAPOR PRESSURE:N/A
SPECIFIC GRAVITY: N/A
SOLUBILITY IN WATER: N/A

L-Lactate Standard

PHYSICAL STATE / FORM: Liquid
COLOR: NONE
ODOR:NONE
pH FACTOR:(20°C)around 7
VISCOSITY:(20°C)N/A
MELTING POINT:N/A
BOILING POINT:N/A
IGNITION TEMPERATURE:N/A
FLASHPOINT:N/A
EXPLOSION LEVEL:LOWER: N/A
UPPER: N/A
VAPOR PRESSURE:N/A
SPECIFIC GRAVITY: N/A
SOLUBILITY IN WATER: N/A

SECTION X:STABILITY AND REACTIVITY

SUBSTANCES TO BE AVOIDED: N/A
DANGEROUS REACTIONS: No dangerous reactions known.
HAZARDOUS, COMBUSTION, OR DECOMPOSITION PRODUCTS: No dangerous decomposition products known.

SECTION XI:TOXICOLOGICAL INFORMATION

TOXICITY DATA: N/A
ACUTE EFFECTS INHALATION: N/A
EYE CONTACT: May cause eye irritation.
SKIN CONTACT: May cause eye irritation.
INGESTION: May be harmful if swallowed.
PROLONGED EXPOSURE: N/A
CHRONIC EFFECTS: N/A
RTECS number: N/A
ADDITIONAL INFORMATION: The product should be handled with routine precautionary measures for handling chemicals.Additional harmful properties cannot be ruled out.

SECTION XII:ECOLOGICAL INFORMATION

No information available.

SECTION XIII:DISPOSAL CONSIDERATIONS

Chemical waste generators must determine whether a discarded chemical is classified as a hazardous waste.
US EPA guidelines for the classification determination are listed in 40 CFR Parts 261.3.
Additionally, waste generators must consult state and local hazardous waste regulations to ensure complete and accurate classification.

SECTION XIV:TRANSPORT INFORMATION

DOT REGULATIONS: Not applicable.

SECTION XV:REGULATORY INFORMATION

No information available.

SECTION XVI:OTHER INFORMATION


The information above is believed to be accurate and represents the best information currently available to us. However, we make no warranty of merchantability or any other warranty, express or implied, with respect to such information, and we assume no liability resulting from its use. Users should make their own investigations to determine the suitability of the information for their particular purposes. Eton Bioscience Inc. shall not be held liable for any damages or other consequences resulting from handling or from contact with the above product.

Q. What type of medium should I use for making cultured cells for Lactate assay?
A. Please use phenol red free medium. Please do not use phenol red medium since phenol red would affectabsorbance readings.

Q. What enzymes is used in Lactate Assay?
A. It is LDH.

Q. Can you tell me the compositions of your Lactate Assay?
A. Unfortunately it is proprietary.

Q. What type of anticoagulant should I use for my samples?
A.If you are collecting blood for measuring lactate from serum ,you do not have to collect blood using an anticoagulant.If you are collecting blood for measuring lactate from blood, you can use heparin or EDTA.

Q. How do I know whether I need to use L-Lactate or D-Lactate assay kit?
A. L-Lactate assay kits are commonly used for measuring lactate level in animal cells.
D-lactate assay kits are commonly used for measuring lactate level in bacteria cells.

Q. Do I need to make a standard curve everytime?
A. Yes, it is necessary since you calculate your sample concentration based on the standard curve. Please do not usethe representative Lactate standard curve in the protocol to calculate concentrations of your samples.

Q.Can I use fluorescence spectroscopy to measure readings for Lactate assay kits?
A. No, the kit does not work with a fluorescence reader since the kit employs colorimetric assays.

Q. How do I measure Lactate from cultured cells?
A. You can grow cells in phenol red-free medium, then you can extract cells with 80% ethanol.

Q. Can I use frozen samples?
A. Although it is better to use fresh samples for assays, you canuse frozen samples. If you are not using frozen samples in once, please make aliquots of your samples before you put them in a freezer to prevent from degrading samples from repeated freeze-thaw cycles.
Q. My sample formed preciptation after adding acetic acid. How can I prevent this?
A. You can still measure readings without adding acetic acid.
Q. The protocol recommends measuring the absorbance at 490nm. Is 485nm close enough?
A. It is ok as long as you set up tje absorbance between 470 and 490nm.
Q. Will L-Lactate assay measure D-Lactate?
A. No, it wont.
Q. Can I use a transparent wall 96-well plate with Lactate Kit?
A. Yes, you can.

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2. Jun Mifune, Katrin Grage, and Bernd H. A. Rehm.:Production of Functionalized Biopolyester Granules by Recombinant Lactococcus lactis.Appl. Envir. Microbiol., Jul 2009; 75: 4668 - 4675.


3. Dragutin J. Savic, William M. McShan, and William M. McShan.:Long-term survival of Streptococcus pyogenes in rich media is pH-dependent.Microbiology, Jun 2012; 158: 1428 - 1436


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5.Janina P. Lewis, Divya Iyer, and Cecilia Anaya-Bergman.:Adaptation of Porphyromonas gingivalis to microaerophilic conditions involves increased consumption of formate and reduced utilization of lactate.Microbiology, Nov 2009; 155: 3758 - 3774.


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7.Inna Serganova, Asif Rizwan, Xiaohui Ni, Sunitha B. Thakur, Jelena Vider, James Russell, Ronald Blasberg, and Jason A. Koutcher.:Metabolic Imaging: A Link between Lactate Dehydrogenase A, Lactate, and Tumor Phenotype.Clin. Cancer Res., Oct 2011; 17: 6250 - 6261.


8.Changlu Liu, Jiejun Wu, Jessica Zhu, Chester Kuei, Jingxue Yu, Jonathan Shelton, Steven W. Sutton, Xiaorong Li, Su Jin Yun, Taraneh Mirzadegan, Curt Mazur, Fredrik Kamme, and Timothy W. Lovenberg.:METABOLISM, REGULATION, AND SIGNALING: Lactate Inhibits Lipolysis in Fat Cells through Activation of an Orphan G-protein-coupled Receptor, GPR81.J. Biol. Chem., Jan 2009; 284: 2811 - 2822.


9.Marina Ostroukhova, Nicholas Goplen, Md Zunayet Karim, Lidia Michalec, Lei Guo, Qiaoling Liang, and Rafeul Alam.:The role of low-level lactate production in airway inflammation in asthma.Am J Physiol Lung Cell Mol Physiol, Feb 2012; 302: L300 - L307.



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5820 Oberlin Drive, Suite 108
San Diego, CA 92121
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