Mouse Myoblast (C2C12) Cells

C2C12 cells are a mouse myoblast cell line. Under appropriate conditions, these cells differentiate into contractile myotubes and produce characteristic muscle proteins. Treatment with bone morphogenic protein 2 (BMP-2) causes a shift in the differentiation pathway from myoblastic to osteoblastic.This product is available for US/Canada customers only.

Sku #Product NameProduct SizePriceQTY
2800100012 Mouse Myoblast (C2C12) Cells 1 ml 1 ml $219.00 USD
 

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1 vial of Mous Myloblast(C2C12) Cells
1 mL, >2×106 cells/mL in 70% DMEM, 20% FBS, 10% DMSO


► shipped on dry ice and stored in liquid nitrogen vapor phase
► When received, the cryovial containing cells should be immediately placed in liquid nitrogen

a. Thawing:
1. Prewarm complete culture medium.
2. Take out the cryovial and immediately thaw in a 37.0°C water bath.
3. Transfer cells from the vial to a T75 culture flask (or a 60 mm cell culture petri dish) and add 10 ml complete medium.
4. Replace the medium 24 hours later.
5. Change medium every 2-3 days.
6. Important: proceed to subcultivation (see below for protocol) before cells reach 80% confluence, unless differentiation is desired.
b. Subculture:
1. Remove and discard culture medium.
2. Briefly rinse the cell layer with prewarmed PBS.
3. Remove PBS.
4. Add 2.0 ml of Trypsin-EDTA solution to flask and incubate at 37.0°C.
5. Gently agitate the cells to detach all cells from the substrate.
6. Observe cells under an inverted microscope
7. Add 8.0 ml of complete growth medium and gently pipet up and down for 20 times.
8. Add appropriate aliquots of the cell suspension to new culture vessels.
9. Incubate cultures at 37°C.
  1. Culture medium and passage information
Subcultivation Ratio: A subcultivation ratio of 1:20 is recommended
Differentiation:
1. Culture cells till they reach 100% confluence.
2. Replace culture medium with differentiation medium.
3. Three days later, replace differentiation medium every 3 days up to 2 weeks.
Culture Medium: Dulbecco's Modified Eagle Medium + 20% FBS + 100 U/ml penicillin-streptomycin
Differentiation Medium: Culture medium + 2% horse serum
Culture Temperature: 37.0°C
Culture Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Freeze medium: Complete growth medium supplemented with 10% (v/v) DMSO

  1. Can I make a stable cell line for commercial purposes?
No.
  1. Is the concentration indicated for cell line products guaranteed?
We guarantee the indicated concentration or higher when we froze down the cells. We routinely check viability of our cells after freezing and make sure that cells are good at our hands. However, it is hard to guarantee the indicated viable cell number because of shipping and handling process, as sometimes unpredictable things happen. However, if the majority of the cells do not survive after thawing, please email products@etonbio.com.

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San Diego, CA 92121
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